circRNA basic information
circBase ID: hsa_circ_0011496
Name: hsa_circ_CSMD2
Synonym: circ_0011496 / circCSMD2
Host Gene: CSMD2
Genomic location(hg19): chr1:34164352-34209207:-
Genomic location(hg38): chr1:33698752-33743606:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung
Cell lines:

BEL-7404 (7404); HCC-LM3 (LM3); HepG2; HLF; HUVEC
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: TWF1, MMP9, VEGF, Ago2
Associated microRNA: miR-486-5p
Biological function: promotes lung fibroblast activation/CAF transformation, enhances fibroblast motility, promotes angiogenesis and recruits HCC cell invasion to facilitate lung-specific metastasis
Molecular mechanism: Exosomal circ_0011496 acts as a miRNA sponge for miR-486-5p in recipient lung fibroblasts, leading to derepression of TWF1 and activation of a TWF1/MMP9 cascade and VEGF-associated crosstalk.
Biological pathway or process:

ceRNA regulation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); metastasis (promotes); fibrosis (promotes); inflammation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); IHC (Immunohistochemistry); RNA Pull-Down; Northern Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; H&E Staining
Clinical significance:

-
Description:

In B[a]P-exposed HCC models, circ_0011496 (circCSMD2) is up-regulated and enriched in tumor-derived exosomes. After delivery to lung fibroblasts, it sponges miR-486-5p, increases TWF1 and downstream MMP9/VEGF signals, promoting CAF-like activation, angiogenesis, and lung-specific metastasis.
Confidence score:

0.848
Other information
Title:

Exposure of benzo[a]pyrene induces HCC exosome-circular RNA to activate lung fibroblasts and trigger organotropic metastasis.

Journal: Cancer communications (London, England)
Published: 2024
PubMed ID: 38840551
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -