circRNA basic information
circBase ID: hsa_circ_0085121
Name: hsa_circ_RNF19A
Synonym: circRNF19A
Host Gene: RNF19A
Genomic location(hg19): chr8:101276261-101300495:-
Genomic location(hg38): chr8:100264033-100288267:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

prostate cancer tissues; adjacent normal prostate tissues; tumor tissues; paracancerous tissues
Cell lines:

22Rv1; LNCaP; PC3; DU145; LN95; RWPE-1; HEK293T; 293T; PC-3; VCaP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HSP90AA1, HNRNPF, AR, AR-V7
Associated microRNA: -
Biological function: Promotes proliferation, invasion, migration, anti-apoptotic capacity, and docetaxel resistance of prostate cancer cells; promotes AR activation and AR-V7 generation and CRPC phenotypes.
Molecular mechanism: Encodes a protein (circRNF19A-490aa) that binds HSP90AA1 to enhance AR activity and activate Akt/mTOR and PLK1 pathways; binds HNRNPF to recruit it to the AR-V7 splice site to enhance AR-V7 alternative splicing; AR transcriptionally regulates RNF19A to modulate circRNF19A expression.
Biological pathway or process:

PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); drug resistance (promotes); cell cycle (promotes); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; TUNEL; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Co-IP; ChIP / ChIP-seq; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis
Clinical significance:

circRNF19A was highly expressed in prostate cancer tissues and was strongly correlated with an advanced Gleason score, biochemical recurrence and advanced T stage.
Description:

hsa_circ_0085121 (circRNF19A), derived from RNF19A, is up-regulated in prostate cancer tissues and cell lines and correlates with aggressive clinicopathological features. It encodes a 490-aa protein that promotes malignant phenotypes and drug resistance by interacting with HSP90AA1 to enhance AR nuclear activity and activate Akt/mTOR and PLK1 signaling, and by interacting with HNRNPF to facilitate AR-V7 alternative splicing and CRPC-related phenotypes.
Confidence score:

0.8949
Other information
Title:

Androgen-targeted hsa_circ_0085121 encodes a novel protein and improves the development of prostate cancer through facilitating the activity of PI3K/Akt/mTOR pathway and enhancing AR-V7 alternative splicing.

Journal: Cell death & disease
Published: 2024
PubMed ID: 39567496
Study type:

combined biological and clinical study
Data availability: PRJNA1052995; TCGA_PRAD
Code availability: -