| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha, VEGFA |
| Associated microRNA: |
miR-140-3p |
| Biological function: |
Promotes endothelial cell proliferation, migration, and angiogenesis in CRC microenvironment; potential diagnostic biomarker in CRC serum |
| Molecular mechanism: |
Exosomal circ_0084043 acts as a miR-140-3p sponge to de-repress HIF-1alpha and increase VEGFA expression/secretion, activating VEGF signaling in endothelial cells |
| Biological pathway or process: |
angiogenesis (promotes); HIF-1 signaling (promotes); VEGF/VEGFR (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; ELISA; CCK8; Transwell Assay; Tube Formation Assay |
| Clinical significance: |
Elevated in CRC patient serum; diagnostic biomarker (ROC AUC 0.93); associated with positive lymph node metastasis and advanced tumor stages |
| Description: |
Exosomal circ_0084043 is up-regulated in CRC patient serum and is enriched in CAF-derived exosomes. After transfer into endothelial cells, it promotes proliferation/migration and angiogenesis by sponging miR-140-3p, thereby increasing HIF-1alpha and VEGFA and enhancing VEGF signaling; it also shows strong diagnostic performance (AUC 0.93). |
| Confidence score: |
0.7126 |