| Expression pattern: |
UP |
| Associated gene: |
NSUN2, YBX1, AKT1 |
| Associated microRNA: |
miR-1301-3p |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion |
| Molecular mechanism: |
NSUN2-mediated m5C methylation enhances circRNA stability; YBX1 binds hsa_circ_0004516; hsa_circ_0004516 binds miR-1301-3p and relieves repression of AKT1, activating AKT signaling |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); m5A modification (other); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
novel therapeutic target and biomarker for BCBM |
| Description: |
hsa_circ_0004516 is upregulated in breast cancer brain metastasis and promotes 231-BR cell proliferation, migration, and invasion by activating AKT signaling. NSUN2-dependent m5C methylation increases its stability, with YBX1 binding as an m5C reader, and a ceRNA relationship is supported via miR-1301-3p targeting AKT1. |
| Confidence score: |
0.7968 |