circRNA basic information
circBase ID: hsa_circ_0009043
Name: -
Synonym: circ_0009043 / circRNA_0009043
Host Gene: -
Genomic location(hg19): chr2:72945231-72960247:-
Genomic location(hg38): chr2:72718102-72733118:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissues / normal lung tissues; xenograft tumors
Cell lines:

A549; HCC827; HCI-H1299; NCI-H1568; NCI-H1650; HCI-H358; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: DNAJB4
Associated microRNA: miR-148a-3p
Biological function: inhibits proliferation; promotes apoptosis; suppresses tumor growth
Molecular mechanism: ceRNA sponge of miR-148a-3p leading to DNAJB4 upregulation
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circ_0009043 expression was predictive of poorer NSCLC patient overall survival.
Description:

circ_0009043 is down-regulated in NSCLC tissues/cells and acts as a tumor-suppressive circRNA. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-148a-3p, thereby relieving repression of DNAJB4 and inhibiting proliferation while promoting apoptosis and reducing xenograft tumor growth.
Confidence score:

0.8426
Other information
Title:

CircRNA 0009043 suppresses non-small-cell lung cancer development via targeting the miR-148a-3p/DNAJB4 axis.

Journal: Biomarker research
Published: 2022
PubMed ID: 35974419
Study type:

combined biological and clinical study
Data availability: The data and study materials that support the findings of this study will be available to other researchers from the corresponding authors on reasonable request.
Code availability: -