| Expression pattern: |
UP |
| Associated gene: |
ERBB3, Ago2 |
| Associated microRNA: |
miR-145 |
| Biological function: |
promotes proliferation, migration, and invasion of HER2-positive breast cancer cells |
| Molecular mechanism: |
ceRNA mechanism: circEPSTI1 sponges miR-145 to regulate ERBB3 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circEPSTI1 could be used as a novel biomarker and potential therapeutic target for breast cancer. |
| Description: |
circEPSTI1 is up-regulated in HER2-positive breast cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes proliferation, migration and invasion by acting as a ceRNA sponge for miR-145, thereby relieving repression of ERBB3. |
| Confidence score: |
0.7785 |