circRNA basic information
circBase ID: hsa_circ_0002360
Name: hsa_circ_RUNX1
Synonym: circ_0002360
Host Gene: -
Genomic location(hg19): chr21:36206706-36231875:-
Genomic location(hg38): chr21:34834409-34859578:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor specimens; adjacent lung samples

Cell lines:

H1299/Taxol; A549/Taxol; H1299; A549; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: G protein regulated inducer of neurite outgrowth 1 (GPRIN1), Ago2
Associated microRNA: miR-585-3p
Biological function: promotes Taxol resistance, proliferation, motility and invasiveness; inhibits apoptosis
Molecular mechanism: ceRNA sponging of miR-585-3p to modulate GPRIN1
Biological pathway or process:

chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation

Clinical significance:

circ_0002360 expression was correlated with tumor size, differentiation, and lymphatic metastasis of the tumors

Description:

circ_0002360 is up-regulated in Taxol-resistant NSCLC and mainly localized in the cytoplasm. It promotes Taxol resistance and malignant behaviors (proliferation, migration/invasion) and suppresses apoptosis by sponging miR-585-3p to increase GPRIN1 expression, and circ_0002360 knockdown sensitizes tumors to Taxol in a xenograft model.

Confidence score:

0.8443

Other information
Title:

Circular RNA circ_0002360 regulates the Taxol resistance and malignant behaviors of Taxol-resistant non-small cell lung cancer cells by microRNA-585-3p-dependent modulation of G protein regulated inducer of neurite outgrowth 1.

Journal: Bioengineered
Published: 2022
PubMed ID: 35293280
Study type:

combined biological and clinical study

Data availability: GSE158695; GSE112214
Code availability: -