| Expression pattern: |
UP |
| Associated gene: |
ACTL6A mRNA, AGO2 |
| Associated microRNA: |
miR-1200 |
| Biological function: |
promotes HCC cell viability/proliferation, colony formation, migration, invasion, EMT, and xenograft tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circ_0084615 sponges miR-1200 to relieve repression of ACTL6A, increasing ACTL6A expression and promoting malignant phenotypes/EMT |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
Upregulated in HCC tissues and positively correlated with TNM staging; potential diagnostic biomarker and therapeutic target |
| Description: |
circ_0084615 is up-regulated in HCC and promotes proliferation, migration, invasion and EMT, as well as xenograft tumor growth. It functions mainly in the cytoplasm as a miRNA sponge for miR-1200, relieving repression of ACTL6A and increasing ACTL6A expression. |
| Confidence score: |
0.8037 |