| Expression pattern: |
UP |
| Associated gene: |
WDR1 |
| Associated microRNA: |
miR-518c-3p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, EMT and tumour growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ-RPL15 acts through a ceRNA-like mechanism by targeting miR-518c-3p and regulating WDR1 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; Survival Analysis; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
Elevated circ-RPL15 in NSCLC was linked with poor prognosis, and high expression was associated with tumour size, T stage, N stage, clinical stage and lower 5-year survival rate. |
| Description: |
circ-RPL15 is up-regulated in NSCLC tissues and cell lines and its high expression is associated with worse 5-year survival. Functionally, circ-RPL15 promotes NSCLC cell proliferation, migration, invasion, EMT and xenograft tumour growth while suppressing apoptosis. Mechanistically, it targets miR-518c-3p to regulate WDR1 expression, forming a circ-RPL15/miR-518c-3p/WDR1 oncogenic axis. |
| Confidence score: |
0.8202 |