circRNA basic information
circBase ID: hsa_circ_0006749
Name: hsa_circ_EIF4A3
Synonym: circEIF4A3 / circ-EIF4A3
Host Gene: EIF4A3
Genomic location(hg19): chr17:78109802-78112079:-
Genomic location(hg38): chr17:80136003-80138280:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue (xenograft)
Cell lines:

NCM460; SW480; HCT116; HCT8; SW620; DLD-1; HEK293; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EIF4A3, ATG5, ATG7, USP14, Ago2
Associated microRNA: miR-3126-5p
Biological function: promotes CRC cell growth/proliferation and tumorigenesis; promotes autophagy; inhibits apoptosis
Molecular mechanism: ceRNA sponging of miR-3126-5p to upregulate EIF4A3; positive feedback where EIF4A3 promotes circEIF4A3 formation; EIF4A3 stabilizes ATG5 mRNA and stabilizes USP14 mRNA to enhance ATG7 protein stability
Biological pathway or process:

autophagy (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; TUNEL; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

circEIF4A3 (hsa_circ_0006749) is upregulated in colorectal cancer cells and promotes proliferation/tumor growth while reducing apoptosis. Mechanistically, it sponges miR-3126-5p to increase EIF4A3, forming a positive feedback loop in which EIF4A3 also enhances circEIF4A3 biogenesis; elevated EIF4A3 promotes autophagy by stabilizing ATG5 mRNA and USP14 mRNA to increase ATG7 protein stability.
Confidence score:

0.8378
Other information
Title:

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Journal: Translational oncology
Published: 2024
PubMed ID: 38795560
Study type:

biological research
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.; Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.tranon.2024.101996.
Code availability: -