| Expression pattern: |
UP |
| Associated gene: |
WDR12, Ago2 |
| Associated microRNA: |
miR-544 |
| Biological function: |
Aggravates myocardial ischemia-reperfusion injury by exacerbating oxidative stress and inflammation and reducing cardiomyocyte viability. |
| Molecular mechanism: |
ceRNA sponging: circMIRIAF sponges miR-544 to relieve inhibition on WDR12, affecting WDR12/Notch1 signaling and downstream oxidative stress/inflammation markers. |
| Biological pathway or process: |
Notch (inhibits); oxidative phosphorylation (unclear); inflammation (promotes); NF-kappaB (promotes); other pathway/process (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circMIRIAF can serve as a drug target for treating MI/RI. |
| Description: |
circMIRIAF (hsa_circ_0006174; RAD23B-derived) is up-regulated in cardiomyocytes under H/RI and in MI/RI models. It functions as a ceRNA by sponging miR-544, thereby increasing WDR12 and modulating the WDR12/Notch1-associated oxidative stress and inflammatory response to aggravate injury; circMIRIAF silencing alleviates these effects. |
| Confidence score: |
0.6993 |