| Expression pattern: |
UP |
| Associated gene: |
SHMT1, TGF-alpha |
| Associated microRNA: |
miR-198 |
| Biological function: |
hsa_circ_0025036 promotes lung adenocarcinoma cell proliferation and inhibits apoptosis; knockdown suppresses proliferation and promotes apoptosis in A549 and Calu-3 cells. |
| Molecular mechanism: |
hsa_circ_0025036 probably acts as a competing endogenous RNA and molecular sponge of miR-198, regulating the miR-198 downstream targets SHMT1 and TGF-alpha. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0025036 expression is associated with aggressive clinicopathological characteristics including differentiation state, TNM stage and lymph node metastasis, and may serve as a potential prognostic biomarker and therapeutic target. |
| Description: |
hsa_circ_0025036 is up-regulated in lung adenocarcinoma tissues and correlates with aggressive clinicopathological characteristics. Functionally, it promotes proliferation and suppresses apoptosis in lung adenocarcinoma cells, probably through a ceRNA-like hsa_circ_0025036/miR-198/SHMT1 and TGF-alpha axis. The study suggests hsa_circ_0025036 as a potential prognostic biomarker and therapeutic target. |
| Confidence score: |
0.7968 |