| Expression pattern: |
DN |
| Associated gene: |
large tumor suppressor kinase 2 (LATS2) |
| Associated microRNA: |
miR-654-3p |
| Biological function: |
Suppresses gastric cancer cell viability, proliferation, invasion, EMT, glycolysis, and in vivo tumor growth. |
| Molecular mechanism: |
circ_0067514 acts as a ceRNA/miRNA sponge for miR-654-3p, thereby regulating LATS2 expression and suppressing gastric cancer progression and glycolysis. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); EMT (inhibits); glycolysis (inhibits); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); ELISA |
| Clinical significance: |
circ_0067514 expression was correlated with gastric cancer overall survival, lymph node metastasis, TNM stage, and histological differentiation; low circ_0067514 expression was associated with poor overall survival. |
| Description: |
hsa_circ_0067514 is down-regulated in gastric cancer tissues and cells, and low expression is associated with poor overall survival and adverse clinicopathological features. Functionally, circ_0067514 suppresses gastric cancer proliferation, invasion, EMT, glycolysis, and xenograft tumor growth. Mechanistically, it acts as a miR-654-3p sponge to upregulate LATS2, forming a circ_0067514/miR-654-3p/LATS2 axis. |
| Confidence score: |
0.8489 |