| Expression pattern: |
UP |
| Associated gene: |
VEGFA, Beclin1, p62 |
| Associated microRNA: |
miR-5047 |
| Biological function: |
Promotes cervical cancer cell invasion, metastasis-associated lymphatic vessel formation, cisplatin chemoresistance, and cell viability under cisplatin treatment; inhibits apoptosis under cisplatin treatment. |
| Molecular mechanism: |
hsa_circ_0023404 directly interacts with miR-5047 and regulates VEGFA expression; it also enhances cisplatin chemoresistance through autophagy signaling. |
| Biological pathway or process: |
ceRNA regulation (other); invasion (promotes); metastasis (promotes); angiogenesis (promotes); autophagy (other); apoptosis (inhibits); chemoresistance (promotes); drug resistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Transwell Assay; Tube Formation Assay; MTT; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
hsa_circ_0023404 was upregulated in cervical tumors and positively correlated with VEGFA; the hsa_circ_0023404/miR-5047/VEGFA axis was suggested to play a critical clinical role in cervical tumor. |
| Description: |
hsa_circ_0023404 is upregulated in cervical cancer tissues and functions as a pro-metastatic and pro-chemoresistance circRNA. It directly interacts with miR-5047, is linked to regulation of VEGFA, promotes invasion and lymphatic vessel formation, and enhances cisplatin resistance through autophagy-related signaling and reduced apoptosis. |
| Confidence score: |
0.6536 |