circRNA basic information
circBase ID: -
Name: hsa_circ_N4BP2L2
Synonym: circRNA NEDD4 binding protein 2 like 2 / circ-N4BP2L2
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue samples; matching adjacent normal tissues; NSCLC tumor tissues; tumor tissues from nude mice
Cell lines:

H1650; PG49; H1299; A549; HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ARL5B, ADP-ribosylation factorlike 5B, Ago2, Cyt-c, Caspase-9, Caspase-3, c-IAP, Drp1, FIS1, MFN1
Associated microRNA: miR-135a-5p
Biological function: circ-N4BP2L2 promotes NSCLC cell proliferation, invasion, mitochondrial function and tumor growth, and inhibits apoptosis and mitochondrial fission-related dysfunction.
Molecular mechanism: circ-N4BP2L2 acts as a ceRNA by sponging miR-135a-5p, thereby relieving miR-135a-5p-mediated suppression of ARL5B and enhancing mitochondrial function in NSCLC cells.
Biological pathway or process:

proliferation (promotes); invasion (promotes); apoptosis (inhibits); mitochondrial function (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; TUNEL; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); FISH / smFISH; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ-N4BP2L2 expression predicted unfavorable prognosis and was linked to advanced clinical stage and positive lymph node metastasis in NSCLC patients.
Description:

circ-N4BP2L2 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with advanced clinical stage, lymph node metastasis, and poor prognosis. Functionally, it promotes NSCLC proliferation, invasion, mitochondrial function, and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ-N4BP2L2 sponges miR-135a-5p to increase ARL5B expression, forming a circ-N4BP2L2/miR-135a-5p/ARL5B ceRNA axis.
Confidence score:

0.8148
Other information
Title:

Circ-N4BP2L2 enhances mitochondrial function in non-small cell lung cancer cells through regulating the miR-135a-5p/ARL5B axis.

Journal: Environmental toxicology
Published: 2023
PubMed ID: 36637163
Study type:

combined biological and clinical study
Data availability: -
Code availability: -