| Expression pattern: |
UP |
| Associated gene: |
HSF1, Ago2 |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
Promotes cervical cancer cell proliferation, migration and invasion and inhibits apoptosis; promotes xenograft tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-615-5p to upregulate HSF1; miR-615-5p directly targets HSF1 3'UTR; rescue by miR-615-5p inhibition or HSF1 overexpression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; RIP (RNA Immunoprecipitation); FISH / smFISH; Luciferase Reporter Assay; Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
High circ_0006789 expression was correlated with tumor differentiation and TNM stage. |
| Description: |
circ_0006789 (circSLC25A43) is upregulated in cervical cancer tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion; inhibits apoptosis) and xenograft tumor growth. Mechanistically, it mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-615-5p to increase HSF1 expression, with rescue by miR-615-5p inhibition or HSF1 overexpression. |
| Confidence score: |
0.8069 |