| Expression pattern: |
UP |
| Associated gene: |
SOX4, ALKBH5 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
promotes cell proliferation; promotes cell invasion; promotes cell cycle progression; promotes tumor growth |
| Molecular mechanism: |
ALKBH5-mediated m6A modification regulates circFOXP1; circFOXP1 sponges miR-338-3p to regulate SOX4 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; Western Blot |
| Clinical significance: |
High circFOXP1 expression was positively associated with tumor size, lymph node metastasis, TNM stage, and poor prognosis in patients with GC; higher circFOXP1 expression was an independent risk factor for DFS and OS |
| Description: |
circFOXP1 is up-regulated in gastric cancer and its high expression correlates with aggressive clinicopathologic features and poor survival. Functionally, circFOXP1 promotes proliferation, invasion, and cell-cycle progression and enhances tumor growth in vivo. Mechanistically, ALKBH5-mediated m6A modification regulates circFOXP1, which acts as a ceRNA by sponging miR-338-3p to increase SOX4 expression. |
| Confidence score: |
0.834 |