circRNA basic information
circBase ID: -
Name: -
Synonym: circ_001422
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum samples; CRC cell-derived exosomes; cells; exosomes
Cell lines:

HCT-116; Caco2; HUVEC; HT29; SW480
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: KDR, PI3K, AKT, mTOR
Associated microRNA: miR-195-5p
Biological function: Promotes endothelial cell proliferation, migration, and angiogenesis/tube formation through exosomal transfer from aggressive CRC cells; serves as a potential CRC diagnostic biomarker.
Molecular mechanism: Exosomal circ_001422 acts as a miR-195-5p sponge, relieving miR-195-5p-mediated repression of KDR and activating PI3K/AKT/mTOR signaling in endothelial cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); angiogenesis (promotes); PI3K/AKT/mTOR (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Western Blot; Transfection; Transwell Assay; Tube Formation Assay
Clinical significance:

Up-regulated in CRC sera; associated with advanced TNM stage and lymph node metastasis; ROC AUC = 0.92; proposed as a biomarker for CRC diagnosis and potential therapeutic target.
Description:

circ_001422 is up-regulated in CRC serum, CRC cells, and CRC-derived exosomes and has diagnostic relevance, including association with advanced stage and lymph node metastasis. Mechanistically, exosomal circ_001422 from aggressive CRC cells is transferred to endothelial cells, where it sponges miR-195-5p, increases KDR expression, activates PI3K/AKT/mTOR signaling, and promotes endothelial proliferation, migration, and angiogenesis.
Confidence score:

0.7078
Other information
Title:

Colorectal cancer-secreted exosomal circ_001422 plays a role in regulating KDR expression and activating mTOR signaling in endothelial cells by targeting miR-195-5p.

Journal: Journal of cancer research and clinical oncology
Published: 2023
PubMed ID: 37432457
Study type:

combined biological and clinical study
Data availability: The data supporting the findings of this study are available from the corresponding author upon reasonable request; Supplementary material available at https:// doi. org/ 10. 1007/ s00432-023-05095-1
Code availability: -