| Expression pattern: |
UP |
| Associated gene: |
GPX4 |
| Associated microRNA: |
miR-491-5p |
| Biological function: |
promotes NSCLC cell proliferation, metastasis, tumor growth and EMT; suppresses ferroptosis |
| Molecular mechanism: |
circ_0082374 acts as a ceRNA by sponging miR-491-5p to up-regulate GPX4 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ferroptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
suggesting a novel therapeutic approach for NSCLC patients |
| Description: |
circ_0082374 is up-regulated in NSCLC tissues and cells. It promotes NSCLC proliferation, metastasis, EMT and in vivo tumor growth while suppressing ferroptosis. Mechanistically, circ_0082374 functions as a ceRNA that sponges miR-491-5p to increase GPX4 expression. |
| Confidence score: |
0.8006 |