circRNA basic information
circBase ID: hsa_circ_0000190
Name: hsa_circ_CNIH4
Synonym: -
Host Gene: CNIH4
Genomic location(hg19): chr1:224553580-224559125:+
Genomic location(hg38): chr1:224365878-224371423:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor tissues; normal tissue samples
Cell lines:

A549; H460; HBE1; 293T; A549/DDP; H460/DDP
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: IL-6, Ago2
Associated microRNA: miR-1253
Biological function: promotes cisplatin (DDP) chemoresistance; enhances DDP IC50; knockdown increases DDP sensitivity
Molecular mechanism: Acts as a miRNA sponge for miR-1253, thereby promoting IL-6 upregulation and cisplatin chemoresistance.
Biological pathway or process:

chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay
Clinical significance:

High hsa_circ_0000190 is associated with advanced TNM stage and larger tumor size in NSCLC patients.
Description:

hsa_circ_0000190 is up-regulated in cisplatin-resistant NSCLC tissues and resistant cell sublines and promotes DDP chemoresistance. Mechanistically, it mainly localizes to the cytoplasm and acts as a sponge for miR-1253, relieving repression of IL-6 and thereby increasing IL-6 levels to support resistance.
Confidence score:

0.7726
Other information
Title:

Hsa_circ_0000190 Promotes NSCLC Cell Resistance to Cisplatin via the Modulation of the miR-1253/IL-6 Axis.

Journal: Analytical cellular pathology (Amsterdam)
Published: 2024
PubMed ID: 38440120
Study type:

combined biological and clinical study
Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -