| Expression pattern: |
UP |
| Associated gene: |
HNF1B |
| Associated microRNA: |
miR-217 |
| Biological function: |
promotes proliferation, migration, invasion, EMT, stemness and drug resistance; inhibits apoptosis (under Oxaliplatin treatment) |
| Molecular mechanism: |
ceRNA mechanism: circ50547 sponges miR-217 to upregulate HNF1B expression, promoting gastric cancer progression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); stemness (promotes); chemoresistance (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ROC Analysis; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circ50547 shows high diagnostic effect for GC (AUC 0.90) and is suggested as a biomarker for diagnosis and prognosis prediction |
| Description: |
Exosomal circ50547 (hsa_circ_0050547, from UBA2) is upregulated in gastric cancer tissues and in patient serum exosomes and shows strong diagnostic performance in exosomes. Functionally, circ50547 promotes GC cell proliferation, migration/invasion, EMT, stemness and Oxaliplatin resistance by sponging miR-217 to upregulate HNF1B, enhancing tumor growth in a cell line-derived xenograft model. |
| Confidence score: |
0.8584 |