circRNA basic information
circBase ID: -
Name: hsa_circ_MAN1A2
Synonym: circMAN1A2 / circ-MAN1A2
Host Gene: MAN1A2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer / OC
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue samples; ovarian cancer tissues; normal tissues; subcutaneous neoplasia
Cell lines:

SKOV3; A2780; IOSE-80; HEK 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IL1RAP, TAK1
Associated microRNA: miR-135a-3p
Biological function: promotes proliferation; promotes clone formation; promotes migration; promotes tumorigenicity/tumor growth
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-135a-3p to relieve repression of IL1RAP and promote TAK1 phosphorylation.
Biological pathway or process:

proliferation (promotes); migration (promotes); MAPK (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

may be a biomarker for early detection of OC
Description:

circMAN1A2 is up-regulated in ovarian cancer cells/tissues and promotes proliferation, colony formation, migration, and xenograft tumor growth. Mechanistically, it functions as a ceRNA that sponges miR-135a-3p, thereby increasing IL1RAP and enhancing TAK1 phosphorylation (MAPK-related signaling), which promotes ovarian cancer progression.
Confidence score:

0.8512
Other information
Title:

Circular RNA circMAN1A2 promotes ovarian cancer progression through the microRNA-135a-3p/IL1RAP/TAK1 pathway.

Journal: PeerJ
Published: 2024
PubMed ID: 38680890
Study type:

combined biological and clinical study
Data availability: All the raw data are available in the Supplementary Files.
Code availability: -