| Expression pattern: |
UP |
| Associated gene: |
AGO2, c-MYC |
| Associated microRNA: |
miR-1294 |
| Biological function: |
Promotes HCC cell proliferation, DNA synthesis, migration, invasion, EMT and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circUBAP2 acts as a cytoplasmic miR-1294 sponge to upregulate c-MYC expression, thereby promoting HCC progression and EMT-related malignant phenotypes. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circUBAP2 expression is associated with worse OS and TTR after surgical resection and is an independent prognostic factor; patients with higher expression were more likely to have microvascular invasion and worse differentiation. |
| Description: |
circUBAP2 (hsa_circ_0003945), derived from the human UBAP2 gene, is up-regulated in HCC tissues and high expression predicts poor OS and TTR. Functionally, it promotes proliferation, tumor growth, migration, invasion and EMT while inhibiting apoptosis. Mechanistically, cytoplasmic circUBAP2 sponges miR-1294 to increase c-MYC expression, forming a circUBAP2-miR-1294-c-MYC regulatory axis. |
| Confidence score: |
0.8556 |