circRNA basic information
circBase ID: -
Name: -
Synonym: circ-alphaPD1
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: extracellular
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

HEK-293T; Hepa1-6
In vivo animal model:

syngeneic model
circRNA-disease information
Expression pattern:
UN
Associated gene: anti-PD-1 scFv
Associated microRNA: -
Biological function: Expresses functional anti-PD-1 scFv, reduces PD-1 in tumor tissues and enhances T-cell infiltration/immune response; inhibits tumor progression in vivo.
Molecular mechanism: CircRNA platform for expressing anti-PD-1 scFv that binds PD1 and blocks PD-1/PD-L1 immune checkpoint signaling.
Biological pathway or process:

immune regulation (promotes)
Detected method:
Others
Validation methods:

Transfection; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); ELISA
Clinical significance:

-
Description:

circ-alphaPD1 is an artificial circRNA engineered to express anti-PD-1 scFv. It produces functional scFv that binds PD1, reduces PD-1 signaling in tumors, and promotes antitumor immune responses, contributing to tumor suppression in mouse HCC models.
Confidence score:

0.4838
Other information
Title:

Utilization of artificial circular RNAs as miRNA sponges and anti-PD-1 scFv expression platforms to suppress hepatocellular carcinoma progression.

Journal: Frontiers in immunology
Published: 2025
PubMed ID: 40568595
Study type:

biological research
Data availability: https://www.frontiersin.org/articles/10.3389/fimmu.2025.1609165/full#supplementary-material
Code availability: -