circRNA basic information
circBase ID: hsa_circ_0042880
Name: hsa_circ_NF1
Synonym: circular NF1-419
Host Gene: NF1
Genomic location(hg19): chr17:29483000-29490394:+
Genomic location(hg38): chr17:31155982-31163376:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021636
MONDO name: astrocytic tumor
Disease details: astroglioma
Disease DO ID:
 3069
Disease MeSH ID:
 -
Disease NCIt ID:
 C6958
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

U87; U87-NF1-419
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UN
Associated gene: L-VOCC, PLCbeta3, Mucin1, CAT4, CAT1, AKAP4, PTDSS1, ganosporeric acid A, ganodermatriol, ganoderic acid B, alpha-D-Arabinofuranosyladenine
Associated microRNA: -
Biological function: Inhibits proliferation and tumor growth, induces apoptosis, and regulates lipid metabolism in astroglioma cells.
Molecular mechanism: circNF1-419 regulates lipid metabolism through glycerophospholipid metabolism and retrograde endocannabinoid signaling pathways, modulates key metabolic enzyme proteins, and may be activated by G. lucidum compounds predicted by virtual docking.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); lipid metabolism (other); other pathway/process (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; circRNA-seq; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Cell Cycle Assay; TUNEL; In Vivo Animal Model; ELISA; Western Blot; Bioinformatics Analysis
Clinical significance:

circNF1-419 could be a therapeutic target for the clinical treatment of astroglioma.
Description:

circNF1-419 was selected as a target after G. lucidum extract L3 increased its expression in U87 astroglioma cells. Overexpression of circNF1-419 inhibited astroglioma cell growth and xenograft tumor growth, promoted apoptosis, and altered lipid metabolism through glycerophospholipid metabolism and retrograde endocannabinoid signaling with changes in metabolic regulators including L-VOCC, PLCbeta3, Mucin1, CAT4, CAT1, AKAP4, and PTDSS1. The study proposes circNF1-419 as a potential therapeutic target for astroglioma.
Confidence score:

0.7296
Other information
Title:

Circular RNA NF1-419 Inhibits Proliferation and Induces Apoptosis by Regulating Lipid Metabolism in Astroglioma Cells.

Journal: Recent patents on anti-cancer drug discovery
Published: 2022
PubMed ID: 34376137
Study type:

biological research
Data availability: GSE 113244; Table S1; Supplementary material is available on the publisher's website along with the published article.
Code availability: -