| Expression pattern: |
UP |
| Associated gene: |
EIF3D, RAD51 mRNA |
| Associated microRNA: |
- |
| Biological function: |
promotes radiotherapy resistance and enhances DNA double-strand break repair via homologous recombination |
| Molecular mechanism: |
acts as a scaffold to recruit EIF3D to RAD51 mRNA 5′-UTR, promoting RAD51 translation initiation and enhancing homologous recombination repair |
| Biological pathway or process: |
homologous recombination repair (promotes); ceRNA regulation (other); DNA damage repair (other); drug resistance (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; Colony Formation Assay; IF (Immunofluorescence); Western Blot; Flow Cytometry（Non-apoptosis/cycle）; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
high circCDYL2 expression is associated with poor prognosis and lower overall survival in NPC |
| Description: |
circCDYL2 is up-regulated in nasopharyngeal carcinoma and correlates with poor overall survival and radiotherapy resistance. Mechanistically, it acts as a scaffold binding EIF3D and RAD51 mRNA to promote RAD51 translation initiation, enhancing homologous recombination repair and radioresistance. |
| Confidence score: |
0.89 |