| Expression pattern: |
UP |
| Associated gene: |
PKM2 |
| Associated microRNA: |
miR-122-5p |
| Biological function: |
Promotes PTC proliferation, tumor growth, migration, and pulmonary metastasis; promotes glycolysis. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-122-5p to increase PKM2 expression, thereby enhancing aerobic glycolysis and malignant phenotypes in PTC. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
circPHGDH is a candidate marker for PTC diagnostics and may have a promising role in PTC treatment. |
| Description: |
circPHGDH is up-regulated in papillary thyroid carcinoma tissues and cell lines and mainly localizes to the cytoplasm. Silencing circPHGDH suppresses proliferation, migration, tumor growth and lung metastasis, and reduces glycolysis by acting as a ceRNA for miR-122-5p to regulate PKM2. |
| Confidence score: |
0.697 |