| Expression pattern: |
DN |
| Associated gene: |
ATG14, PIK3C3, Beclin-1, LC3B, AKT-mTOR pathway |
| Associated microRNA: |
miR-1825 |
| Biological function: |
Suppresses proliferation and migration; promotes autophagy; inhibits CRC progression and metastasis |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1825 to restore/upregulate ATG14 expression, regulating autophagy; inhibits AKT-mTOR pathway activation |
| Biological pathway or process: |
autophagy (promotes); proliferation (inhibits); migration (inhibits); invasion (other); metastasis (inhibits); PI3K/AKT/mTOR (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNase R Treatment; RT-qPCR; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circTBC1D22A is down-regulated in colorectal cancer tissues and functions as a tumor suppressor. It sponges miR-1825 to restore ATG14 expression, promoting autophagy and thereby inhibiting CRC cell proliferation, migration, and metastasis; it also counteracts AKT-mTOR activation. |
| Confidence score: |
0.7949 |