| Expression pattern: |
UP |
| Associated gene: |
ODC1, ornithine decarboxylase 1 |
| Associated microRNA: |
miR-498 |
| Biological function: |
promotes HCC cell proliferation, migration, invasion, metastasis, EMT and tumor growth; inhibits apoptosis |
| Molecular mechanism: |
circ_0003028 acts as a ceRNA sponge for miR-498 to regulate ODC1 expression and can be encapsulated in exosomes to promote EMT |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
promising biomarker and therapeutic target for HCC |
| Description: |
circ_0003028 is up-regulated in HCC tissues, cells and patient plasma/serum exosomes. It promotes HCC proliferation, migration, invasion, EMT and in vivo tumor growth while suppressing apoptosis, mainly through a circ_0003028/miR-498/ODC1 ceRNA axis and exosome-mediated EMT promotion. The study suggests circ_0003028 may serve as a biomarker and therapeutic target for HCC. |
| Confidence score: |
0.8175 |