| Expression pattern: |
DN |
| Associated gene: |
CPEB3, p53 mRNA |
| Associated microRNA: |
miR-889-3p |
| Biological function: |
Suppresses bladder cancer malignant proliferation and tumor growth; promotes apoptosis; induces G0/G1 arrest. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-889-3p to regulate CPEB3, which stabilizes p53 mRNA via binding to the CPE signal in p53 3′UTR. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other); p53 signaling (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; divergent primers PCR; Sanger Sequencing; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Low circKDM1A expression is significantly associated with poor prognosis of BCa; circKDM1A may be a potential prognostic biomarker and therapeutic target of BCa. |
| Description: |
circKDM1A (hsa_circ_0009061) is down-regulated in bladder cancer and acts as a tumor suppressor by inhibiting proliferation and tumor growth while promoting apoptosis. Mechanistically, it sponges miR-889-3p to relieve repression of CPEB3, and CPEB3 stabilizes p53 mRNA via binding the CPE signal in p53 3′UTR. |
| Confidence score: |
0.8574 |