| Expression pattern: |
DN |
| Associated gene: |
GRB2, FOXO1, FOXO3 |
| Associated microRNA: |
miR-182-5p, miR-520q-3p, miR-582-3p, miR-587, miR-655 |
| Biological function: |
Inhibits NSCLC cell proliferation and invasion; enhances sensitivity to Taxol; suppresses tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-182-5p (and other miRNAs) to regulate GRB2, FOXO1, and FOXO3 expression, affecting NSCLC progression and Taxol sensitivity |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); drug resistance (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; Western Blot |
| Clinical significance: |
Decreased circ_0002483 correlated with poor prognosis in NSCLC; patients with low circ_0002483 had worse prognosis than those with high expression |
| Description: |
circ_0002483 is down-regulated in NSCLC tissues/cell lines and acts as a tumor suppressor by inhibiting proliferation and invasion. It enhances Taxol sensitivity by sponging miR-182-5p (and other miRNAs), thereby increasing GRB2/FOXO1/FOXO3 expression; higher circ_0002483 expression is associated with better patient prognosis. |
| Confidence score: |
0.8295 |