| Expression pattern: |
DN |
| Associated gene: |
PVT1 |
| Associated microRNA: |
miR-1208, miR-1825, miR-197, miR-203, miR-339-3p, miR-526b, miR-1827 |
| Biological function: |
potential regulatory role in GC at the posttranscriptional level |
| Molecular mechanism: |
predicted circRNA-miRNA-mRNA regulatory network (potential ceRNA mechanism) |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
diagnostic biomarker for GC; AUC 0.792 in tissues and 0.872 in whole-blood; combined use with CEA/CA199/CA125 AUC 0.933; negatively correlated with tumor depth and lymph node metastasis |
| Description: |
hsa_circ_0001821 (host gene PVT1) is significantly down-regulated in gastric cancer tissues and in patients’ whole blood, and shows cytoplasmic enrichment, suggesting a post-transcriptional regulatory role. It has diagnostic potential for GC (ROC AUC up to 0.872 in blood; improved to 0.933 when combined with CEA/CA199/CA125) and is associated with tumor depth and lymph node metastasis. A circRNA-miRNA-mRNA network involving seven predicted miRNAs was proposed but not experimentally validated. |
| Confidence score: |
0.6981 |