| Expression pattern: |
UP |
| Associated gene: |
CNBP, CDK6 mRNA |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration and invasion; suppresses apoptosis |
| Molecular mechanism: |
recruits CNBP to promote binding to CDK6 mRNA, enhancing CDK6 mRNA stability and translation |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Sanger Sequencing; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; TUNEL; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
- |
| Description: |
circPRMT5 is up-regulated in osteosarcoma cell lines and mainly localized in the cytoplasm. It promotes proliferation, migration and invasion and inhibits apoptosis by recruiting the RBP CNBP to enhance CDK6 mRNA stability and translation, thereby increasing CDK6 and malignant activity. |
| Confidence score: |
0.8 |