| Expression pattern: |
DN |
| Associated gene: |
HIF-1alpha, p-ERK1/2, p-AKT, E-cadherin, ZEB1 |
| Associated microRNA: |
- |
| Biological function: |
circ-EPHB4 acts as a tumor inhibitor in HCC; its overexpression suppresses cell viability and proliferation, induces apoptosis, inhibits migration and invasion, and reduces tumorigenesis and metastasis in vivo. |
| Molecular mechanism: |
circ-EPHB4 inhibits HIF-1alpha expression and suppresses HIF-1alpha-related signaling, including PI3K-AKT and the HIF-1alpha-ZEB1 axis. |
| Biological pathway or process: |
HIF-1 signaling (inhibits); PI3K/AKT (inhibits); ERK (inhibits); EMT (inhibits); proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; ROC Analysis; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; RNA-seq; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-EPHB4 is a candidate biomarker of HCC; ROC analysis showed high sensitivity and specificity, and low circ-EPHB4 correlated with positive HBsAg and vascular tumor thrombus. |
| Description: |
circ-EPHB4 (hsa_circ_0001730), derived from EPHB4, is downregulated in HCC tissues and shows biomarker potential by ROC analysis. Functionally, circ-EPHB4 suppresses HCC cell proliferation, migration, invasion, tumorigenesis and metastasis while promoting apoptosis. Mechanistically, its antitumor effect is mediated through inhibition of HIF-1alpha expression and downstream suppression of HIF-1alpha-related PI3K-AKT and ZEB1/EMT signaling. |
| Confidence score: |
0.7944 |