| Expression pattern: |
UP |
| Associated gene: |
cyclin D1, CCND1 |
| Associated microRNA: |
miR-134 / miR-134-5p |
| Biological function: |
Promotes LAC cell proliferation, migration and invasion; inhibits cell apoptosis; associated with TNM stage and lymphatic metastasis; potential non-invasive biomarker for early detection and screening of LAC. |
| Molecular mechanism: |
hsa_circ_0013958 acts as a cytoplasmic miR-134 sponge and up-regulates oncogenic CCND1/cyclin D1 protein in LAC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0013958 levels were associated with TNM stage and lymphatic metastasis, and showed diagnostic accuracy for LAC with tissue AUC 0.815 and plasma AUC 0.794; it may serve as a potential non-invasive biomarker for early detection and screening of LAC. |
| Description: |
hsa_circ_0013958 is up-regulated in LAC tissues, cell lines and plasma, and its expression is associated with TNM stage and lymphatic metastasis. Functionally, it promotes proliferation, migration and invasion while inhibiting apoptosis in LAC cells. Mechanistically, cytoplasmic hsa_circ_0013958 sponges miR-134 and up-regulates oncogenic CCND1/cyclin D1, supporting its value as a potential non-invasive biomarker for LAC detection. |
| Confidence score: |
0.8419 |