| Expression pattern: |
DN |
| Associated gene: |
QKI |
| Associated microRNA: |
miR-224-5p, miR-1250-3p, miR-338-5p |
| Biological function: |
Inhibits cadmium-induced transformation of BEAS-2B cells; inhibits proliferation, EMT, invasion, migration, and anchorage-independent growth; promotes QKI expression. |
| Molecular mechanism: |
circ-SHPRH acts as a miR-224-5p sponge to regulate QKI expression, and QKI and circ-SHPRH form a positive-feedback loop under cadmium treatment. |
| Biological pathway or process: |
proliferation (inhibits); EMT (inhibits); invasion (inhibits); migration (inhibits); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ-SHPRH is down-regulated during the middle and late stages of cadmium-induced transformation of human bronchial epithelial BEAS-2B cells. Functionally, circ-SHPRH suppresses proliferation, EMT, migration, invasion, and anchorage-independent growth by sponging miR-224-5p to restore QKI expression. The study also supports a positive-feedback loop between QKI and circ-SHPRH under cadmium treatment, suggesting circ-SHPRH as a potential target for preventing or treating cadmium-induced lung cancer. |
| Confidence score: |
0.717 |