| Expression pattern: |
DN |
| Associated gene: |
PTRF, Ago2 |
| Associated microRNA: |
miR-1197 |
| Biological function: |
Suppresses osteosarcoma cell proliferation and invasion and inhibits tumor growth in vivo (tumor-suppressive). |
| Molecular mechanism: |
Acts as a competing endogenous RNA (ceRNA) by sponging miR-1197, thereby relieving miR-1197-mediated repression of PTRF. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0049271 is down-regulated in osteosarcoma tissues and cell lines and mainly localized in the cytoplasm. It suppresses OS cell proliferation and invasion and inhibits xenograft tumor growth by acting as a ceRNA that sponges miR-1197 to restore PTRF expression (circ_0049271/miR-1197/PTRF axis). |
| Confidence score: |
0.7795 |