| Expression pattern: |
UP |
| Associated gene: |
eIF4A3, STAU1, TPI1, PRDX6 |
| Associated microRNA: |
- |
| Biological function: |
promotes migration and proliferation of ESCC |
| Molecular mechanism: |
circ-231 acts as a molecular scaffold to promote eIF4A3-STAU1 interaction and facilitate unwinding of 5′ UTR secondary structure of TPI1/PRDX6 mRNAs, enhancing translation (protein biogenesis) without changing mRNA levels |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); mRNA stability (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Western Blot; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; Transwell Assay; Colony Formation Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
higher expression of circ-231 correlates with lymph node metastasis risk in ESCC |
| Description: |
In ESCC, circ-231 is up-regulated and promotes proliferation and migration. Mechanistically, it serves as a scaffold that enhances eIF4A3-STAU1 interaction, enabling unwinding of 5′ UTR secondary structures in TPI1/PRDX6 mRNAs and increasing their protein production without changing mRNA levels, and its high expression associates with lymph node metastasis risk. |
| Confidence score: |
0.6993 |