| Expression pattern: |
UP |
| Associated gene: |
MYCBP, AGO2 |
| Associated microRNA: |
miR-889-3p |
| Biological function: |
Promotes osteosarcoma cell proliferation, migration, invasion, tumor growth and lung metastasis; inhibits apoptosis. |
| Molecular mechanism: |
Directly binds MYCBP to prevent ubiquitin-mediated proteasome degradation (K11-linked ubiquitination) and functions as a ceRNA sponge for miR-889-3p to increase MYCBP mRNA/protein, enhancing c-MYC transcriptional activity and downstream genes (CCND1, c-Jun, CDK4). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ubiquitination (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0002669 is associated with unfavorable overall survival and shows diagnostic value (AUC=0.87). |
| Description: |
circ_0002669 is upregulated in osteosarcoma and is associated with poor overall survival. It promotes OS proliferation, migration/invasion and tumor growth/metastasis by stabilizing MYCBP protein via inhibiting ubiquitin-proteasome degradation and by sponging miR-889-3p to increase MYCBP expression, enhancing c-MYC transcriptional activity. |
| Confidence score: |
0.8933 |