circRNA basic information
circBase ID: hsa_circ_0031242
Name: hsa_circ_PRMT5
Synonym: circRNA protein arginine methyltransferase-5 / circ-PRMT5
Host Gene: PRMT5
Genomic location(hg19): chr14:23389732-23392044:-
Genomic location(hg38): chr14:22920523-22922835:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

DDP-resistant NSCLC tissues; DDP-sensitive NSCLC tissues; xenograft tumor tissues

Cell lines:

HBE1; A549; H1299; A549/DDP; H1299/DDP

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MYH9, P-gp, MRP1, IL-2, TNF-alpha, TGF-beta
Associated microRNA: miR-138-5p
Biological function: Circ-PRMT5 promotes cisplatin resistance, cell viability, migration and invasion, inhibits apoptosis, suppresses T-cell immune response, and supports tumor growth in DDP-resistant NSCLC.
Molecular mechanism: Circ-PRMT5 acts as a ceRNA/molecular sponge for miR-138-5p to regulate MYH9 expression, thereby modulating DDP resistance and immune response in NSCLC.
Biological pathway or process:

chemoresistance (promotes); drug resistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); immune regulation (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; ELISA; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; Bioinformatics Analysis

Clinical significance:

Circ-PRMT5 may provide a promising circRNA-targeted therapy for NSCLC with DDP resistance.

Description:

Circ-PRMT5 is up-regulated in DDP-resistant NSCLC tissues and cells. Its knockdown increases cisplatin sensitivity and T-cell immune response, suppresses malignant phenotypes and tumor growth, and acts mechanistically through the miR-138-5p/MYH9 axis.

Confidence score:

0.6542

Other information
Title:

Circular RNA PRMT5 knockdown enhances cisplatin sensitivity and immune response in non-small cell lung cancer by regulating miR-138-5p/MYH9 axis.

Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology
Published: 2021
PubMed ID: 34761592
Study type:

combined biological and clinical study

Data availability: -
Code availability: -