circRNA basic information
circBase ID: hsa_circ_0000598
Name: hsa_circ_B2M
Synonym: circ_001680
Host Gene: B2M
Genomic location(hg19): chr15:45009906-45009989:+
Genomic location(hg38): chr15:44717708-44717791:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent paracancerous normal colorectal tissues
Cell lines:

FHC; HCT116; SW480; HCT15; SW620; CACO2; DLD1; LOVO; HT29; HCT8; RKO; SW837
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: BMI1, AGO2
Associated microRNA: miR-340
Biological function: promotes proliferation and migration; promotes CSC population/stemness; induces irinotecan therapeutic resistance; inhibits apoptosis
Molecular mechanism: Acts as a miR-340 sponge and upregulates BMI1 (ceRNA mechanism); associates with AGO2-containing RISC
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); stemness (promotes); chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining
Clinical significance:

circ_001680 may be a promising diagnostic and prognostic marker to determine the success of irinotecan-based chemotherapy.
Description:

In colorectal cancer, circ_001680 is up-regulated and promotes proliferation, migration, tumorigenicity, and CSC-like properties. Mechanistically, it sponges miR-340 to relieve repression of BMI1, thereby enhancing stemness and inducing irinotecan resistance.
Confidence score:

0.815
Other information
Title:

Hsa_circ_001680 affects the proliferation and migration of CRC and mediates its chemoresistance by regulating BMI1 through miR-340.

Journal: Molecular cancer
Published: 2020
PubMed ID: 32005118
Study type:

combined biological and clinical study
Data availability: All data generated or analysed during this study are included in this published article and its Additional files.
Code availability: -