circRNA basic information
circBase ID: hsa_circ_0013561
Name: hsa_circ_SLC16A1
Synonym: circ-SLC16A1 / circ-solute carrier family 16 member 1
Host Gene: SLC16A1
Genomic location(hg19): chr1:113459799-113460666:-
Genomic location(hg38): chr1:112917177-112918044:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; para-carcinoma tissue; matched adjacent nontumorous tissues; tumor tissues; lung tissues; plantar lymphoid tissue

Cell lines:

A549; HCC1833; BEAS-2B; Luc-Hcc1833

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: PFN2
Associated microRNA: miR-1287-5p
Biological function: promotes proliferation; promotes migration; promotes invasion; promotes metastasis; inhibits apoptosis; promotes cell cycle progression
Molecular mechanism: circ-SLC16A1 sponges miR-1287-5p to increase PFN2 expression (ceRNA mechanism).
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes)

Detected method:
Q
H
S
Validation methods:

RNA-seq; FISH / smFISH; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis

Clinical significance:

High expression of circ-SLC16A1 portends poor prognosis; negatively correlated with survival; positively correlated with lymphatic metastasis and TNM classification.

Description:

circ-SLC16A1 (hsa_circ_0013561) is up-regulated in NSCLC tissues/cells and mainly localized in the cytoplasm. It promotes NSCLC proliferation, migration, invasion and metastasis while inhibiting apoptosis and facilitating cell-cycle progression. Mechanistically, it functions as a miRNA sponge for miR-1287-5p to increase PFN2 expression, and high circ-SLC16A1 expression is associated with poorer patient survival and more advanced disease features.

Confidence score:

0.84

Other information
Title:

Oncogenic circ-SLC16A1 promotes progression of non-small cell lung cancer via regulation of the miR-1287-5p/profilin 2 axis.

Journal: Cellular & molecular biology letters
Published: 2024
PubMed ID: 38539084
Study type:

combined biological and clinical study

Data availability: All data in this study are available.
Code availability: -