| Expression pattern: |
DN |
| Associated gene: |
BNIP3, BCL2 interacting protein 3 |
| Associated microRNA: |
miR-1178-3p |
| Biological function: |
CircDUSP22 overexpression restrains pancreatic cancer cell proliferation and colony formation, arrests the cell cycle at G0/G1 phase, promotes apoptosis, and decelerates tumor growth in vivo. |
| Molecular mechanism: |
CircDUSP22 functions as a miR-1178-3p sponge to increase BNIP3 expression, thereby inhibiting PaCa development. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNase R Treatment; RT-qPCR; RNA-seq; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
CircDUSP22 may be a potential targeted therapy strategy for PaCa. |
| Description: |
circDUSP22/hsa_circ_0075410 is down-regulated in pancreatic cancer tissues and cell lines and is mainly localized in the cytoplasm. Its overexpression suppresses PaCa cell growth, induces cell-cycle arrest and apoptosis, and reduces xenograft tumor growth. Mechanistically, circDUSP22 sponges miR-1178-3p to upregulate BNIP3, supporting its potential as a therapeutic target in PaCa. |
| Confidence score: |
0.8256 |