| Expression pattern: |
UP |
| Associated gene: |
MUC1 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes proliferation and migration of breast cancer cells |
| Molecular mechanism: |
circ_0009910/miR-145-5p/MUC1 axis; circ_0009910 negatively correlates with miR-145-5p and its silencing increases miR-145-5p and decreases MUC1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Wound Healing Assay; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Up-regulation of circ_0009910 in breast tumors is related to invasion of the tumor to lymph node; circ_0009910 can be suggested as an appreciated biomarker for breast cancer (AUC = 0.7544; P value < 0.0001) with 78% sensitivity and 76% specificity |
| Description: |
circ_0009910 is up-regulated in breast cancer tissues and supports oncogenic phenotypes. siRNA knockdown of circ_0009910 in MCF-7 cells reduces proliferation and migration, increases miR-145-5p, and decreases MUC1, supporting a circ_0009910/miR-145-5p/MUC1 regulatory axis. Clinically, higher circ_0009910 is associated with lymph node invasion and shows diagnostic potential by ROC analysis. |
| Confidence score: |
0.743 |