| Expression pattern: |
UP |
| Associated gene: |
PABPC1, eIF4G, OSMR, HIF1A |
| Associated microRNA: |
- |
| Biological function: |
promotes NSCLC metastasis by inducing macrophage polarization toward M2 type |
| Molecular mechanism: |
acts as a scaffold enhancing PABPC1-eIF4G interaction to promote OSMR translation, activating OSMR/JAK/STAT3 signaling and M2 polarization |
| Biological pathway or process: |
metastasis (promotes); migration (promotes); invasion (promotes); JAK/STAT (promotes); immune regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Co-IP; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Wound Healing Assay; Western Blot; FISH / smFISH; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Cohort Study; Clinical Sample Validation |
| Clinical significance: |
Higher circPLEKHM1 levels are associated with bone metastasis risk and correlate with shorter overall survival and metastasis-free survival in NSCLC patients. |
| Description: |
circPLEKHM1 is a hypoxia-induced circRNA enriched in NSCLC-derived exosomes. It promotes NSCLC metastasis by being transferred to macrophages and acting as a scaffold that enhances PABPC1-eIF4G interaction, boosting OSMR translation and activating OSMR/JAK/STAT3 signaling to drive M2 polarization. High tumor circPLEKHM1 associates with metastasis and poor survival, and circPLEKHM1-ASO treatment reduces metastasis in vivo. |
| Confidence score: |
0.8956 |