| Expression pattern: |
UP |
| Associated gene: |
MYC, NOTCH1, Ago2 |
| Associated microRNA: |
miR-449c-5p |
| Biological function: |
Promotes GC cell migration, invasion, tumor growth, metastasis and stemness; circ-NOTCH1 knockdown inhibits migration, invasion, spheroid formation, side population ratio and stemness-related protein expression. |
| Molecular mechanism: |
Circ-NOTCH1 functions as a miR-449c-5p sponge to regulate MYC, and MYC transcriptionally activates NOTCH1 and circ-NOTCH1, forming a circ-NOTCH1/miR-449c-5p/MYC/NOTCH1 regulatory axis. |
| Biological pathway or process: |
Notch (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-NOTCH1 level is correlated with unfavorable prognosis of patients with GC; circ-NOTCH1 may be a therapeutic marker or target for GC. |
| Description: |
Circ-NOTCH1 is an up-regulated NOTCH1-derived circRNA in gastric cancer and high expression is associated with unfavorable prognosis. It promotes GC growth, migration, invasion, metastasis and stemness by sponging miR-449c-5p to regulate MYC and NOTCH1, forming a circ-NOTCH1/miR-449c-5p/MYC/NOTCH1 regulatory axis. |
| Confidence score: |
0.8816 |