| Expression pattern: |
UP |
| Associated gene: |
HuR, beta-TrCP, USP1, Vimentin |
| Associated microRNA: |
- |
| Biological function: |
promotes GC cell viability/proliferation, migration, invasion, and tumor growth/metastasis |
| Molecular mechanism: |
circUSP1 binds HuR (RRM1 major) and stabilizes HuR protein by inhibiting beta-TrCP-mediated ubiquitination/proteasomal degradation, thereby enabling HuR to post-transcriptionally upregulate USP1 and Vimentin via their 3′ UTR AREs. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Survival Analysis; ROC Analysis |
| Clinical significance: |
Tissue-derived circUSP1 is an independent prognostic factor for GC; plasma-derived circUSP1 shows diagnostic value (ROC AUC 0.726) and outperforms AFP/CEA/CA19-9. |
| Description: |
circUSP1 is upregulated in gastric cancer tissues/cells and promotes proliferation, invasion/metastasis and tumor growth. It predominantly localizes in the cytoplasm, binds HuR (mainly via RRM1) and stabilizes HuR by inhibiting beta-TrCP-mediated ubiquitination, thereby increasing HuR-dependent post-transcriptional upregulation of USP1 and Vimentin and contributing to poor prognosis and diagnostic potential in plasma. |
| Confidence score: |
0.9018 |