| Expression pattern: |
UP |
| Associated gene: |
TLR4 |
| Associated microRNA: |
miR-671-5p |
| Biological function: |
Promotes RA-FLS proliferation, migration, invasion and inflammatory response, and inhibits apoptosis. |
| Molecular mechanism: |
circ-PTTG1IP acts as a miRNA sponge for miR-671-5p and upregulates TLR4 expression through the miR-671-5p/TLR4 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); inflammation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; ELISA; Western Blot |
| Clinical significance: |
circ-PTTG1IP was overexpressed in RA patient synovial tissues and may be a novel target for the treatment of RA. |
| Description: |
circ-PTTG1IP is up-regulated in RA synovial tissues and RA-FLSs. It promotes RA-FLS proliferation, migration, invasion and inflammatory cytokine secretion while suppressing apoptosis, mainly by acting as a cytoplasmic sponge for miR-671-5p to upregulate TLR4 expression. |
| Confidence score: |
0.742 |