| Expression pattern: |
UP |
| Associated gene: |
LAMC2 |
| Associated microRNA: |
miR-548c-3p |
| Biological function: |
promotes HCC proliferation, migration/invasion, metastasis, and EMT; its silencing suppresses these phenotypes |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge for miR-548c-3p to increase LAMC2 expression, thereby promoting EMT and malignant phenotypes in HCC |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
High hsa_circ_0101145 expression was correlated with aggressive HCC phenotypes and predicted poor prognosis. |
| Description: |
hsa_circ_0101145 is up-regulated in HCC tissues and cell lines and mainly localizes to the cytoplasm. It promotes HCC proliferation, migration/invasion, metastasis and EMT by sponging miR-548c-3p, thereby increasing LAMC2 expression; silencing it suppresses tumor growth and metastasis in vitro and in vivo. |
| Confidence score: |
0.7302 |