| Expression pattern: |
UP |
| Associated gene: |
PGK1, VIMENTIN, EIF4A3 |
| Associated microRNA: |
miR-1343-3p |
| Biological function: |
Promotes GC cell proliferation, migration, invasion, tumor growth and metastasis; promotes macrophage M2 polarization. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1343-3p to upregulate PGK1 and activate PI3K/AKT signaling; binds VIMENTIN to reduce Ser83 phosphorylation; packaged into exosomes to mediate cell-cell communication; EIF4A3 promotes circGLIS3 biogenesis by binding flanking sequences of GLIS3 pre-mRNA. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); macrophage polarization (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; circRIP; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
High circGLIS3 expression was associated with advanced TNM stage and lymph node metastasis in GC patients. |
| Description: |
circGLIS3 (hsa_circ_0002874) is up-regulated in gastric cancer and promotes tumor growth and metastasis. It sponges miR-1343-3p to upregulate PGK1 and activate PI3K/AKT signaling, and it also binds VIMENTIN to reduce Ser83 phosphorylation. circGLIS3 is transported via exosomes to neighboring cancer cells and macrophages, enhancing invasion and inducing M2 polarization; EIF4A3 promotes its biogenesis. |
| Confidence score: |
0.8546 |