circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: CircHIPK3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma / ESCC
Disease DO ID:
3748
Disease MeSH ID:
C562729; D000077277
Disease NCIt ID:
C4024
Disease ICD11 ID:
1417891145
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tumor tissues; adjacent normal epithelial tissues

Cell lines:

KYSE140; KYSE510; KYSE150; ECa109; EC9706; HET-1a; 293 T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: FASN
Associated microRNA: miR-637
Biological function: promotes cell proliferation, colony formation, migration, and invasion in vitro; promotes tumor growth in vivo; promotes fatty acid metabolism
Molecular mechanism: circHIPK3 acts as a ceRNA to sponge miR-637, relieving repression of FASN and thereby regulating fatty acid metabolism in ESCC cells
Biological pathway or process:

fatty acid metabolism (promotes); proliferation (promotes); migration (promotes); invasion (promotes)

Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Oil Red O Staining; Western Blot; In Vivo Animal Model

Clinical significance:

may be a potential biomarker and therapeutic target for ESCC in the clinic

Description:

circHIPK3 is up-regulated in ESCC tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion) and xenograft tumor growth. It functions mainly in the cytoplasm as a ceRNA that sponges miR-637 to de-repress FASN, enhancing fatty acid metabolism; ASO-mediated circHIPK3 inhibition suppresses ESCC, suggesting therapeutic potential.

Confidence score:

0.7966

Other information
Title:

CircHIPK3 regulates fatty acid metabolism through miR-637/FASN axis to promote esophageal squamous cell carcinoma.

Journal: Cell death discovery
Published: 2024
PubMed ID: 38431720
Study type:

combined biological and clinical study

Data availability: All the data supporting the findings of this study are available within the article and its additional files and from the corresponding author upon reasonable request.
Code availability: -