circRNA basic information
circBase ID: hsa_circ_0006990
Name: hsa_circ_VAPA
Synonym: circVAPA
Host Gene: VAPA
Genomic location(hg19): chr18:9931806-9937063:+
Genomic location(hg38): chr18:9931809-9937066:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Human NSCLC tissues; matched normal tissues; adjacent non-tumor tissues
Cell lines:

H1299; H1975; A549; HCC827; BEAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: WNT5A, beta-catenin, c-Myc, cyclin D1
Associated microRNA: miR-876-5p
Biological function: Promotes NSCLC cell proliferation, migration, invasion, stemness maintenance and tumor growth in vivo.
Molecular mechanism: CircVAPA functions as a competing endogenous RNA that sponges miR-876-5p, up-regulates WNT5A, and activates Wnt/beta-catenin signaling.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); ceRNA regulation (promotes); apoptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; MTT; EdU Staining; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High expression of circVAPA indicated a poor prognosis in patients with NSCLC; circVAPA may be a potential therapeutic target for NSCLC.
Description:

hsa_circ_0006990, named circVAPA, is up-regulated in NSCLC tissues and cell lines and high expression predicts poor prognosis. It promotes malignant NSCLC phenotypes by sponging miR-876-5p to increase WNT5A expression, thereby activating Wnt/beta-catenin signaling.
Confidence score:

0.817
Other information
Title:

CircVAPA exerts oncogenic property in non-small cell lung cancer by the miR-876-5p/WNT5A axis.

Journal: The journal of gene medicine
Published: 2021
PubMed ID: 33619796
Study type:

combined biological and clinical study
Data availability: The datasets used during the present study are available from the corresponding author upon reasonable request.
Code availability: -